High-Sensitivity Detection of Fruit Tree Viruses Using Bacterial Magnetic Particles
| 作 者：Chen JF, Li Y, Wang ZF,Li SH*, et al |
| 刊物名称：Journal of Integrative Plant Biology |
| 出版年份：2009 |
| 卷：51 |
| 期：4 |
| 页码：409-413 |
论文摘要：
Prunus necrotic ring spot virus (PNRSV) and grapevine fanleaf virus (GFLV) were detected by fluoroimmunoassay using bacterial magnetic particles (BMPs), and a double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA). For the fluoroimmunoassay, fluorescein isothiocyanate labeled anti-PNRSV antibody or anti-GFLV antibody was conjugated onto BMPs of Magnetospirillum gryphiswaldense MSR-1. With this method, a very low minimum antigen concentration (1 x 10(6) dilution of the original sample concentration) could be detected. Using DAS-ELISA, the minimum antigen detection concentration was the original sample concentration. Thus, comparing these two methods, a BMP-based method could increase the sensitivity up to six orders of magnitude (10(6)) higher than an ELISA-based method of detection PNRSV and GFLV.
