Exploring multifunctional components from Andrographis paniculata by affinity ultrafiltration with three molecular targets
| 作 者：Huixia Feng, Guilin Chen, Mingquan Guo* |
| 刊物名称：Food Chemistry |
| 标识符：10.1016/j.foodchem.2022.134515 |
| 出版年份：2023 |
| 卷：404 |
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| 页码：134515 |
论文摘要：
Andrographis paniculata (Burm. F.) Nees (AP) was a typical plant resource that has the concomitant function of both foodstuff and medicine, while the action mechanisms of its immune regulation, anti-inflammatory and antiviral effects and the specific components remain unclear. In this work, a screening approach combining bioaffinity ultrafiltration with liquid chromatography mass spectrometry (UF-LC/MS) was hired to screen potential bioactive compounds from AP. The crude extract of AP exerted COX-2 and ACE2 inhibitory effects by other bioassays. Meanwhile, a total of eleven ligands targeting COX-2, IL-6 and ACE2 were screened out. Thereinto, two compounds including andrographolide and 14-deoxy-11,12-didehydroandrographolide exhibited strong binding affinities to COX-2, IL-6 and ACE2 by UF-LC/MS and molecular docking analysis. This is the first report to apply UF-LC/MS approach to rapidly screen out multi-target ligands from AP, and further decipher corresponding mechanisms, which could be beneficial to expedite the search for new multi-target bioactive compounds in other natural products or foods.
